id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-007461-v3tff2gk	Nguyen, T.D.	Transmissible gastroenteritis (TGE) of swine: In vitro virus attachment and effects of polyanions and polycations	2002-11-12		.txt	text/plain	2901	161	59	Four transmissible gastroenteritis virus (TGEV) strains (Purdue-115, D-52, 188-SG and Gep-II) and two cell lines (swine testis-ST and pig kidney-RPD) were used to study virus attachment and cell susceptibility. A field TGEV strain (Gep-II), which was unable to multiply in cell cultures, appeared able to inhibit the attachment of radiolabelled cell-passaged virus. The effect of these chemicals on virus attachment was tested by using the plaque assay as described above, except that the inoculum containing 50-100 P.F.U. was prepared in the presence of the chemical at appropriate concentrations and incubated with confluent monolayer cells for 30 min at 37 ° C. Differences in susceptibility to TGEV infection, indicated by plaque formation, between the ST and RPD cells and effects of temperature on virus attachment were studied by incubating virus inoculum with the cells for 30 min at either 4 or 37°C.	./cache/cord-007461-v3tff2gk.txt	./txt/cord-007461-v3tff2gk.txt