id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-290788-6y0vjhux	Wang, Qihui	Isolation of Monoclonal Antibodies from Zika Virus-Infected Patient Samples	2020-05-05		.txt	text/plain	4087	359	74	The combination of sorting antigen-specific memory B cells with determining immunoglobulin (Ig) genes at the single-cell level enables the isolation of monoclonal antibodies (mAbs) in individuals. Currently, multiple strategies have been reported to generate human neutralizing mAbs against Zika infection, including sequencing antigen-specific memory B cells [8, 9] or generating Epstein-Barr virus-immortalized memory B cells from Zika patient samples [10] , and identifying functional mAbs from phage display naïve antibody libraries [11] . Here, we introduce a method to apply Zika envelope (E) glycoproteins, which play pivotal roles in virus entry and contain important neutralizing epitopes, to sort single memory B cells from a convalescent Zika patient. Fig. 4 Strategy to clone and express Zika E-specific human mAbs. The Ig genes from the sorted cells were determined and cloned into the expression vectors by a reported approach with some modifications [13, 18] .	./cache/cord-290788-6y0vjhux.txt	./txt/cord-290788-6y0vjhux.txt