id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-346054-k84rcpav	Niespodziana, Katarzyna	PreDicta chip-based high resolution diagnosis of rhinovirus-induced wheeze	2018-06-18		.txt	text/plain	7405	349	47	Here, we develop a chip containing 130 different micro-arrayed RV proteins and peptides and demonstrate in a cohort of 120 pre-school children, most of whom had been hospitalized due to acute wheeze, that it is possible to determine the culprit RV species with a minute blood sample by serology. The analysis of IgG reactivity to structural and non-structural proteins and to recombinant fragments and synthetic peptides spanning VP1, VP2, and VP3 from RV89 is shown in Supplementary Fig. 2a for all 120 children and in Supplementary Fig. 2b for those children (n = 41) who had shown increases of RV89-specific antibody responses in follow-up serum samples taken after recovery. Based on our previous observations that antibody increases specific for the N-terminal portion of VP1 can be detected in serum samples obtained from subjects after RV infection 36 , the PreDicta chip was equipped with a VP1 peptide set which should allow detecting species-specific immune responses at high resolution ( Fig. 1 ).	./cache/cord-346054-k84rcpav.txt	./txt/cord-346054-k84rcpav.txt