id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-103892-v6gkubd4	Mäkinen, Janne J.	The mechanism of the nucleo-sugar selection by multi-subunit RNA polymerases	2020-07-01		.txt	text/plain	8442	418	51	Overall, these data demonstrated that the enhanced or diminished capabilities of the variant RNAPs to utilize 2'dGTP in the SNA assays reflected, in qualitative terms, their capabilities to utilize all four 2'dNTPs. The role of the β'Arg425 in selectively promoting the binding of NTPs was easy to explain because the β'Arg425 interacts with the 2'OH of the NTP analogues in several RNAP structures (Supplementary Table 5 , Fig. 1c, 4a, b) . We further hypothesized and demonstrated by in silico docking experiments that the 3'OH could move to within the hydrogen bond distance of the β'Arg425 if the deoxyribose moiety adopted a 2'-endo conformation (Supplementary Fig. 8 To test this hypothesis in crystallo, we solved the X-ray crystal structure of the initially transcribing complexes containing T. Overall, the comparative analysis of RNAP structures with CMPCPP, 2'dCTP and 3'dCTP suggested that the β'Arg425 inhibited the incorporation of 2'dNTPs by interacting with their 3'OH group and favoring the 2'-endo conformation of the deoxyribose moiety.	./cache/cord-103892-v6gkubd4.txt	./txt/cord-103892-v6gkubd4.txt