id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-103638-n5kpvsvg	Nguyen, Long T.	Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection	2020-04-14		.txt	text/plain	5357	388	60	By extending the 3'or 5'-ends of the crRNA with different lengths of ssDNA, ssRNA, and phosphorothioate ssDNA, we discovered a new self-catalytic behavior and an augmented rate of LbCas12a-mediated collateral cleavage activity as high as 3.5-fold compared to the wild-type crRNA. With isothermal amplification of SARS-CoV-2 RNA using RT-LAMP, the modified crRNAs were incorporated in a paper-based lateral flow assay that could detect the target with up to 23-fold higher sensitivity within 40-60 minutes. However, the ENHANCE showed 5.4-fold and 3.4-fold and higher trans-cleavage activity compared to the wild-type crRNA for targeting the methylated dsDNA and ssDNA, respectively (Fig. 3e, Supplementary Fig. 20a) . While no clinical samples were tested, the results indicated the 3'DNA7-modified crRNA consistently demonstrated higher sensitivity for detecting CoV-2 dsDNA within 30 minutes as compared to the wild-type crCoV-2 ( Supplementary Figs.	./cache/cord-103638-n5kpvsvg.txt	./txt/cord-103638-n5kpvsvg.txt