id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-013614-j6h338qa	Liu, Xiaojing	ERCC6L2 promotes DNA orientation-specific recombination in mammalian cells	2020-04-30		.txt	text/plain	8200	576	55	To identify potentially new NHEJ factors, we combined chemical perturbation screens on 36 compounds with focused CRISPRknockout screens on 414 genes in the CH12 B cell line ( Fig. 1a ; Supplementary information, Table S1 , see Materials and Methods for details). ERCC6L2 clusters with other NHEJ factors Next, we clustered all 414 DNA repair genes by their z-scores across the 36 chemicals used, which categorized genes into three major groups depending on their impact on cell growth (Supplementary information, Fig. S1a ). Furthermore, the helicase catalytic-dead (DEAH > AAAH) mutant did not promote CSR ( Fig. 3a ; Supplementary information, Fig. S4b ), indicating that ERCC6L2's predicted catalytic activity is required for DNA end-joining. To bypass the B cell development defect of core-NHEJ factor deficiencies and quickly access the directional CSR, we deleted the non-productive IgH allele in CH12F3 cells as previous described 18 (Supplementary information, Fig. S8c , named CH12-NCDel cells) and perform HTGTS assay with endogenous AID Sμ baits.	./cache/cord-013614-j6h338qa.txt	./txt/cord-013614-j6h338qa.txt