id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-001270-l8aa9cl3	Wongsrikeao, Pimprapar	Antiviral restriction factor transgenesis in the domestic cat	2011-09-11		.txt	text/plain	6269	333	47	In contrast to primates, feline species lack antiviral TRIM5α genes 11 but have potently restrictive APOBEC3 proteins 9, 10 , which sets up intriguing possibilities for testing such genes at the whole-animal level, for conferring gene-based immunity with them or engineered variants 12, 13 , and potentially for HIV-1 disease model development 10 . In experiments summarized in Supplementary Table 1 , we subjected 195 in vitro-matured grade I and II domestic cat oocytes to perivitelline space microinjection (PVSMI) with lentiviral vector TSinG 5 ; we performed injection 10-12 h before or 10-12 h after in vitro fertilization (IVF) (Supplementary Fig. 1 ). We consistently observed embryo-pervasive, abundant expression of both proteins encoded by dual gene vectors in cat blastocysts when we injected lentiviral vector before IVF ( Fig. 1b and Supplementary Table 2 ).	./cache/cord-001270-l8aa9cl3.txt	./txt/cord-001270-l8aa9cl3.txt