id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-022494-d66rz6dc	Webb, B.	Comparative Modeling of Drug Target Proteins	2014-10-01		.txt	text/plain	8782	453	47	Comparative modeling consists of four main steps 23 (Figure 2 (a)): (1) fold assignment that identifies similarity between the target sequence of interest and at least one known protein structure (the template); (2) alignment of the target sequence and the template(s); (3) building a model based on the alignment with the chosen template(s); and (4) predicting model errors. Modeller implements comparative protein structure modeling by the satisfaction of spatial restraints that include: (1) homologyderived restraints on the distances and dihedral angles in the target sequence, extracted from its alignment with the template structures; 35 (2) stereochemical restraints such as bond length and bond angle preferences, obtained from the CHARMM-22 molecular mechanics force field; 107 (3) statistical preferences for dihedral angles and nonbonded interatomic distances, obtained from a representative set of known protein structures; 108 and (4) optional manually curated restraints, such as those from NMR spectroscopy, rules of secondary structure packing, cross-linking experiments, fluorescence spectroscopy, image reconstruction from electron microscopy, site-directed mutagenesis, and intuition ( Figure 2(b) ).	./cache/cord-022494-d66rz6dc.txt	./txt/cord-022494-d66rz6dc.txt