id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-001786-ybd8hi8y	Dutilh, Bas E	Metagenomic ventures into outer sequence space	2014-12-15		.txt	text/plain	2193	121	44	These are referred to as "unknowns," and reflect the vast unexplored microbial sequence space of our biosphere, also known as "biological dark matter." However, unknowns also exist because metagenomic datasets are not optimally mined. These are referred to as "unknowns," and reflect the vast unexplored microbial sequence space of our biosphere, also known as "biological dark matter." However, unknowns also exist because metagenomic datasets are not optimally mined. Applications include the use of metagenomics for the discovery of novel genetic functionality, 2 for describing microbial ecosystems and tracking their variation, 3 in untargeted medical diagnostics and forensics, 4 and as a powerful tool to determine the genome sequences of rare, uncultivable microbes. The level of unknowns can range up to 99% of the metagenomic reads, depending on the sampled environment, the protocols used for nucleotide isolation and sequencing, the homology search algorithm, and the reference database.	./cache/cord-001786-ybd8hi8y.txt	./txt/cord-001786-ybd8hi8y.txt