id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-353953-83d0g8ix	Mendoza, Emelissa J.	Two Detailed Plaque Assay Protocols for the Quantification of Infectious SARS‐CoV‐2	2020-05-31		.txt	text/plain	4265	281	57	Plaque assays are a quantitative method of measuring infectious SARS‐CoV‐2 by quantifying the plaques formed in cell culture upon infection with serial dilutions of a virus specimen. Plaque assays are a quantitative method of measuring infectious SARS-CoV-2 by quantifying the plaques formed in cell culture upon infection with serial dilutions of a virus specimen (Harcourt et al., 2020) . The first plaque assay method that we describe (Basic Protocol) uses Noble agar as the matrix in a solid overlay and neutral red as the stain to enhance plaque visualization. This protocol outlines a plaque assay method that can be used for the quantification of SARS-CoV-2 plaque-forming units (PFUs) in virus specimens, including viral stocks prepared from infected cell culture supernatants, and with further optimization, bodily fluids from animals infected with SARS-CoV-2. Use Formula 8 (see Basic Protocol) to calculate the titer of SARS-CoV-2 in the specimen using the identified dilution factor and the inoculum volume of 0.1 ml.	./cache/cord-353953-83d0g8ix.txt	./txt/cord-353953-83d0g8ix.txt