id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-353576-f29kmtot	Maricic, T.	A direct RT-qPCR approach to test large numbers of individuals for SARS-CoV-2	2020-06-26		.txt	text/plain	3499	200	60	We then tested 1 l of mouthwash from each of the 20 individuals using two RT-qPCR kits advertised to allow direct detection of SARS-CoV-2 from nasopharyngeal swabs: Luna Universal Probe (NEB, Ipswich, USA) and PrimeDirect (Takara, Kyoto, Japan) as well as another kit, SuperScript III with Platinum Taq (Invitrogen, Waltham, USA). To systematically investigate how the NEB Luna assay performs compared to RNA extraction followed by the Roche assay for mouthwash samples, we investigated 62 gargle lavages from patients that were either negative or presented with various viral loads based on previous investigations. In the first scheme, the samples were tested individually using the direct RT-qPCR protocol and the results were evaluated and reported back to the facility by 7 p.m. To detect any inhibition that the mouthwash samples may introduce into the RT-qPCR reactions, we added a synthesized control RNA that was quantified in parallel with SARS-CoV-2 by a probe .	./cache/cord-353576-f29kmtot.txt	./txt/cord-353576-f29kmtot.txt