id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-352849-vd62r8qu	Artesi, M.	Failure of the cobas(R) SARS-CoV-2 (Roche) E-gene assay is associated with a C-to-T transition at position 26340 of the SARS-CoV-2 genome	2020-05-03		.txt	text/plain	2825	177	61	Control of the ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic requires accurate laboratory testing to identify infected individuals, while also clearing essential staff to continue work. At the current time a number of RT-PCR tests have been developed to identify SARS-CoV-2, targeting multiple regions in the viral genome. Out of the 186 SARS-CoV-2 genomes we have sequenced, only these four samples carry a SNP at position 26340. Vogels et al [17] also identified this 3 base change as well as other SNPs in primer/probe binding sites from a number of RT-PCR assays for SARS-CoV-2. The cobas® E-gene assay may use an alternate primer probe combination that is more sensitive to the presence of the SNP, alternatively it may target the same positions as the Corman et al. . https://doi.org/10.1101/2020.04.28.20083337 doi: medRxiv preprint However, our ability to show that each individual carries a genetically identical virus demonstrates the potential whole genome sequencing has for tracking chains of transmission.	./cache/cord-352849-vd62r8qu.txt	./txt/cord-352849-vd62r8qu.txt