id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-349545-w7c2tu5a	Wang, Mengmeng	Analytical performance evaluation of five RT‐PCR kits for severe acute respiratory syndrome coronavirus 2	2020-10-27		.txt	text/plain	1838	126	55	BACKGROUND: We aimed to evaluate the analytical performance of five commercial RT‐PCR kits (Genekey, Daan, BioGerm, Liferiver, and Yaneng) commonly used in China, since such comparison data are lacking. RESULTS: The positive detection rate was 100% for Genekey, Daan, and BioGerm,and 90% for Liferiver and Yaneng in 20 clinical SARS‐CoV‐2 infection. 8 Pan et al found that thermal inactivation adversely affected the efficiency of RT-PCR for SARS-CoV-2 detection samples with low viral loads. In the work, we presented the analytical performance evaluations of five RT-PCR kits using nasopharyngeal swabs samples from patients with confirmed SARS-CoV-2 infection, and negative nasopharyngeal swabs samples ( Figure 1 ). To further confirm the detection ability of the five kits, a positive clinical specimen (Ct: ORF1ab 26.99, N: 28.19) was diluted with 5-fold using RNase-free water, and the resulting dilution is considered as Level 1. No positive result was obtained in testing of 30 negative clinical samples by using five kits for ORF1ab and N gene.	./cache/cord-349545-w7c2tu5a.txt	./txt/cord-349545-w7c2tu5a.txt