id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-349501-p1fttfpr	Ratia, Kiira	Chapter 494 Coronavirus Papain-like Peptidases	2013-12-31		.txt	text/plain	1322	77	45	Keywords: Coronavirus, Severe acute respiratory syndrome, SARS-CoV, polyprotein processing, ubiquitin-like domain, noncovalent protease inhibitors, de-ubiquitination, DUB, ISG-15, de-ISGylation. For coronaviruses, a family of positive-stranded RNA viruses with large genomes (28À32 kb), the gene encoding the viral non-structural proteins (nsp's), including the RNA-dependent RNA-polymerase, is translated into a large precursor polyprotein, which must be proteolytically processed to mediate viral transcription and replication [1] . Cloning and expression of the N-terminal region of the murine coronavirus replicase polyprotein revealed that a predicted papain-family protease (papain-like protease, PLP) domain was responsible for processing the aminoterminal non-structural protein (nsp) from the replicase polyprotein [2À3] . Analysis of the N-terminal region of the replicase polyprotein of SARS-CoV revealed only one PLP domain, termed PLpro, which was shown to process the nsp1/2, nsp2/3 and nsp3/4 cleavage sites using the LXGG recognition motif [18] . Severe acute respiratory syndrome coronavirus papain-like protease: structure of a viral deubiquitinating enzyme	./cache/cord-349501-p1fttfpr.txt	./txt/cord-349501-p1fttfpr.txt