id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-349365-2ot1kf2k	Shi, Yi	Antisense downregulation of SARS‐CoV gene expression in Vero E6 cells	2004-11-15		.txt	text/plain	3217	175	52	Cells were then treated with the antisense oligonucleotides used by adding them to the culture medium, which then reached the nuclei and resulted in sequence-specific antisense downregulation of SARS-CoV gene expression. We conclude that the antisense PS-ODNs could effectively and sequence-specifically downregulate SARS-CoV gene expression in a dose-dependent manner. In this work, we have evaluated the down-regulation effects of 26 antisense PS-ODNs targeting different sites along the open reading frames (ORFs) of E, M, and N proteins and obtained 12 antisense oligos which could reduce target gene expression by over 50% in Vero E6 cells at the concentration of 50 µM in the culture medium. Our present results indicate a sequencespecific down-regulation effect of antisense PS-ODN (20mer) in Vero E6 cells, and we found an effective range of concentrations, where the antisense oligo inhibited expression of the E, M, and N genes of SARS-CoV in a concentration-dependent manner.	./cache/cord-349365-2ot1kf2k.txt	./txt/cord-349365-2ot1kf2k.txt