id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-347484-7vn93t58	Zamoto, Aya	Identification of Ferret ACE2 and its Receptor Function for Sars-Coronavirus	2006		.txt	text/plain	773	50	61	Severe acute respiratory syndrome associated coronavirus (SARS-CoV) was the causative agent of SARS, which occurred as an emerging pneumonic disease in 2002. Amplification of partial ACE2 gene by RT-PCR: RNAs were extracted from heart, lung, kidney, and small intestine of a ferret. Determination of nucleotide sequence of ferret ACE2 (feACE2): Two overlapping regions of feACE2 genes were amplified by RT-PCR from kidney RNA. The partial feACE2 gene was amplified from RNAs isolated from the lung, heart, kidney, and small intestine by RT-PCR. HeLa cells expressing feACE2 supported SARS-CoV replication to the same extent as those expressing human ACE2 (Fig. 3) . From these observations, it is postulated that animal species whose ACE2 functions as an efficient SARS-CoV receptor would be a susceptible in feACE2 expressing HeLa cells was over 10 times more efficient than that in mouse efficiently. Furthermore, transgenic mouse expressing ferret or human ACE2 may serve a useful animal model for SARS-CoV infection.	./cache/cord-347484-7vn93t58.txt	./txt/cord-347484-7vn93t58.txt