id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-346957-bmajkabp	Lv, Yanbo	Identification of a novel conserved HLA-A*0201-restricted epitope from the spike protein of SARS-CoV	2009-12-03		.txt	text/plain	4933	261	57	RESULTS: First, different SARS-CoV sequences were analyzed to predict eight candidate peptides from conserved regions of the S protein based upon HLA-A*0201 binding and proteosomal cleavage. To investigate the capacity of candidate peptides to mobilize a human CTL repertoire, HLA-A2 + PBLs from ten HLA-A2 + donors were stimulated in vitro by DCs loaded with Alignment of the putative amino acid sequences of S proteins from eighteen SARS-CoV strains A dot among the individual sequences denoted nucleotides that are the same as the consensus. Furthermore, SARS-CoV/S-derived peptides Sp6, Sp7 and Sp8 could not only induce the increased S protein specific IFN-γ secreting T cell frequency but also the enhanced cytolytic capacity of these CTLs. To further address whether the immunogenic candidate peptide is naturally processed and presented, HLA-A2.1/ K b transgenic mice were immunized with S/pVAX1 plasmid containing a full-length cDNA encoding the SARS-CoV/S protein.	./cache/cord-346957-bmajkabp.txt	./txt/cord-346957-bmajkabp.txt