id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-346222-rzbzlnr4	Kim, Dae-Kyum	A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions	2020-08-06		.txt	text/plain	1749	110	48	Here we describe a collection of codon-optimized coding sequences for SARS-CoV-2 cloned into Gateway-compatible entry vectors, which enable rapid transfer into a variety of expression and tagging vectors. SARS-CoV-2 coding sequence collection Gatewaycompatible TEV (tobacco etch virus) sequence A global pandemic of the coronavirus disease COVID-19, a severe respiratory illness caused by a novel virus from the family Coronaviridae (SARS-CoV-2), has infected millions and caused hundreds of thousands of deaths (World Health Organization 2020a). Broad availability of a collection of SARS-CoV-2 CDSs has the potential to enable many downstream biochemical and structural studies and thus a better understanding of processes within the viral life cycle, including scalable assays for screening drug candidates that could potentially disrupt these processes. However, to enable the subsequent removal of such N-terminal fusion tags, we generated an additional set of clones containing, at the N-terminus of the ORF, a recognition sequence for nuclear inclusion protease from tobacco etch virus (TEV).	./cache/cord-346222-rzbzlnr4.txt	./txt/cord-346222-rzbzlnr4.txt