id author title date pages extension mime words sentences flesch summary cache txt cord-345992-3ij1vbqp Drosten, Christian SARS Molecular Detection External Quality Assurance 2004-12-17 .txt text/plain 1746 98 42 Inactivated severe acute respiratory syndrome–associated coronavirus samples were used for an external quality assurance study within the World Health Organization SARS Reference and Verification Network and other reference institutions. Inactivated severe acute respiratory syndrome-associated coronavirus samples were used for an external quality assurance study within the World Health Organization SARS Reference and Verification Network and other reference institutions. Highly sensitive methods for virus detection, such as reverse transcription-polymerase chain reaction (RT-PCR) are required to confirm SARS in the acute phase and prevent transmission. First, laboratories had to correctly detect the four samples containing >9,400 copies of viral RNA per milliliter, a concentration well above the detection limit of published and commercial nucleic acid amplification tests (NAT) for SARS-CoV, (6, 7, (10) (11) (12) . Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-PCR assays ./cache/cord-345992-3ij1vbqp.txt ./txt/cord-345992-3ij1vbqp.txt