id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-343362-4u2re1cu	Liu, Jianjun	SARS Transmission Pattern in Singapore Reassessed by Viral Sequence Variation Analysis	2005-02-22		.txt	text/plain	5408	234	49	METHODS AND FINDINGS: The success rate of the MS-based analysis for detecting SARS coronavirus (SARS-CoV) sequence variations was determined to be 95% with 75 copies of viral RNA per reaction, which is sufficient to directly analyze both clinical and cultured samples. We present here a novel application of mass spectrometry (MS)-based technology in characterizing viral sequence variations that overcomes these problems, and we apply it retrospectively to the severe acute respiratory syndrome (SARS) outbreak in Singapore. The success rate of the MS-based analysis for detecting SARS coronavirus (SARS-CoV) sequence variations was determined to be 95% with 75 copies of viral RNA per reaction, which is sufficient to directly analyze both clinical and cultured samples. A further application of MS-based viral sequence variation analysis in tracking the virus strain and thus the transmission of SARS-CoV was demonstrated by our confirmation of the Singapore origin of a SARS-CoV isolate from a German patient.	./cache/cord-343362-4u2re1cu.txt	./txt/cord-343362-4u2re1cu.txt