id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-343330-wuzts3mt	Ramos da Silva, S.	Broad SARS-CoV-2 cell tropism and immunopathology in lung tissues from fatal COVID-19	2020-09-29		.txt	text/plain	3447	229	56	Background Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) infection in patients with Coronavirus Disease 2019 (COVID-19) prominently manifests with pulmonary symptoms histologically reflected by diffuse alveolar damage (DAD), excess inflammation, pneumocyte hyperplasia and proliferation, and formation of platelet aggregates or thromboemboli. Methods We performed multicolor staining for viral proteins, and lineage cell markers to identify SARS-CoV-2 tropism and to define the lung pathobiology in postmortem tissues from five patients with fatal SARS-CoV-2 infections. Single cell RNA sequencing (scRNA-seq) analysis of lung tissues from healthy 120 subjects have revealed that many cell types express SARS-CoV-2 entry receptor and 121 cofactors including angiotensin-converting enzyme-2 (ACE2), transmembrane serine 122 protease 2 (TMPRSS2), and furin, that are involved in viral entry, suggesting 123 susceptibility of these cells to infection. 7-10 Furthermore, scRNA-seq analysis of 124 bronchoalveolar lavage fluid (BALF), blood, oropharyngeal or lung tissues from COVID-125 19 patients has identified different types of SARS-CoV-2-infected cells, including 126 macrophages, neutrophils, type II pneumocytes (AT2), and ciliated and endothelial 127 cells.	./cache/cord-343330-wuzts3mt.txt	./txt/cord-343330-wuzts3mt.txt