id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-342731-rilr45yb	Donia, Ahmed	RNA interference as a promising treatment against SARS-CoV-2	2020-09-01		.txt	text/plain	844	58	55	Previous study also reported the production of plasmid-mediated small interfering RNAs (siRNAs) to target the viral RNA polymerase, which successfully inhibited the cytopathic effects of SARS-CoV on Vero cells (Wang et al. The genomic and the subgenomic mRNAs of coronaviruses have an identical 5′ leader sequence (via a unique mechanism called discontinuous transcription) and common 3′-ends, a unique feature in coronavirus replication. The siRNA targeting the leader sequence reduced the abundance of mRNA and protein expression levels of the reporter genes in 293 T cell line. They also found that the siRNA targeting the leader sequence could inhibit the replication of SARS-CoV via silencing gene expression in Vero E6 cells. Additionally, siRNA targeting the leader sequence exhibited a much potent inhibitory effect on the replication of SARS-CoV than the siRNAs targeting the spike gene made (Enjuanes et al. ) siRNA targeting the leader sequence of SARS-CoV inhibits virus replication	./cache/cord-342731-rilr45yb.txt	./txt/cord-342731-rilr45yb.txt