id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-342221-xvrpx9p8	Duan, Qing	Reovirus, isolated from SARS patients	2003		.txt	text/plain	1783	103	55	In this report, reovirus was isolated from throat swabs of SARS patients, including the first case in Beijing and her mother. SCV was isolated in lung tissue collected at autopsy from her father, and the first isolate in Beijing was named the BJO 1 strain of SARS-associated coronavirus, whose genome sequence has been determined [4] . Isolation of reovirus: To isolate viruses associated with SARS, we inoculated the clinical specimen (material from throat swabs) obtained from the first patient with SARS admitted to hospital in Beijing onto Hep-2 cells. We isolated the virus in throat swab specimen from the first case in Beijing and her mother, and initially considered it as a possible variant of SCv, but the PCR test failed to amplify DNA from it. Electron microscopy (EM) examination of Hep-2 cells and Vero-E6 cells infected with the virus isolated from throat swab specimens of the first case in Beijing revealed characteristic reovirus particles with a size of about 60-80 urn in diameter.	./cache/cord-342221-xvrpx9p8.txt	./txt/cord-342221-xvrpx9p8.txt