id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-341970-pho6dksc	Huang, Jun	Immunization with SARS-CoV S DNA vaccine generates memory CD4(+) and CD8(+) T cell immune responses	2006-06-05		.txt	text/plain	4417	259	64	In the present study, mice were immunized i.m. with SARS-CoV S DNA vaccine, and three different methods (ELISA, ELISPOT and FACS) were used to evaluate the immune responses when the cells were stimulated in vitro with a pool of peptides overlapping entire SARS spike protein. Moreover, mice boosted with SARS S DNA vaccine exhibited a 3-30-fold increase in the frequency of IFN-␥-producing cells in spleens (P < 0.01) and lymph nodes (P < 0.05), respectively (Fig. 1) , compared with the prime immunization. To further ascertain whether the frequency of SARS-CoV S specific CD4 + and CD8 + T cell responses was increased after boost vaccination, mice were boosted i.m. with SARS-CoV S DNA vaccine, seven days after injection, IFN-␥-and IL-2-producing CD4 + and CD8 + T cells were determined in lymph nodes, spleen and lungs.	./cache/cord-341970-pho6dksc.txt	./txt/cord-341970-pho6dksc.txt