id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-340970-389t032s	Choy, Wai-Yan	Synthetic Peptide Studies on the Severe Acute Respiratory Syndrome (SARS) Coronavirus Spike Glycoprotein: Perspective for SARS Vaccine Development	2004-06-01		.txt	text/plain	3526	172	51	Background: The S (spike) protein of the etiologic coronavirus (CoV) agent of severe acute respiratory syndrome (SARS) plays a central role in mediating viral infection via receptor binding and membrane fusion between the virion and the host cell. Methods: Six peptide sequences corresponding to the surface regions of SARS-CoV S protein were designed and investigated by use of combined bioinformatics and structural analysis. The rabbit and monkey antisera against the synthetic peptides were diluted to 1:40-fold with PBS, and 10 L of each diluted serum was added to a well of the slide that was coated with SARS-CoV-infected African green monkey kidney Vero cells and incubated at 37°C for 1 h. The first batch of the rabbit and monkey antisera against the six synthetic peptides was collected 1 week after the second immunization and was tested for antibody specificity against the corresponding antigen (either conjugatefree or KLH-conjugated peptide) by ELISA analysis.	./cache/cord-340970-389t032s.txt	./txt/cord-340970-389t032s.txt