id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-340635-8wki7noy	Yu, Bin	Innate and adaptive immunity of murine neural stem cell-derived piRNA exosomes/microvesicles against pseudotyped SARS-CoV-2 and HIV-based lentivirus	2020-11-13		.txt	text/plain	6017	300	56	Through testing pseudotyped SARS-CoV-2 and HIV-based lentivirus, this study reports that exosomes/microvesicles (Ex/Mv) isolated from murine hypothalamic neural stem/progenitor cells (htNSC) or subtype htNSCPGHM as well as hippocampal NSC have innate immunity-like actions against these RNA viruses. We then measured some of these piRNAs in NSC Ex/Mv (using htNSC PGHM and J o u r n a l P r e -p r o o f hpNSC as the representative) compared to the levels in MSC Ex/Mv. As shown in Fig. S2B and S3, most of these piRNAs were present in these NSC Ex/Mv but were much less detectable in MSC Ex/Mv. Our additional assays showed that htNSC PGHM were comparable or slightly stronger than htNSC in producing these piRNAs. Thus, based on the information from wildtype and pseudotyped SARS-CoV-2, NSC Ex/Mv contain piRNAs against the genomic sequences of both viruses, although these NSC were not previously exposed to either virus, suggesting that mouse species has evolved to establish large antiviral piRNA libraries in NSC Ex/Mv. We asked if an initial pre-exposure of a specific virus to NSC could lead to an enhancement or enrichment of specific antiviral piRNAs in NSC Ex/Mv. Thus, we treated these NSCs with pseudotyped SARS-CoV-2 virus for 2 generations, and then maintained them under normal culture for about 5 generations.	./cache/cord-340635-8wki7noy.txt	./txt/cord-340635-8wki7noy.txt