id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-340410-s9haq8y1	Fukumoto, Tatsuya	Efficacy of a novel SARS-CoV-2 detection kit without RNA extraction and purification	2020-06-26		.txt	text/plain	1072	81	68	The virus was detected in 53/71 (74.6%) and 55/71 (77.5%) by the direct PCR and nCoV-DK, respectively, with overall concordance rate of 94.4%: 95.2% in nasopharyngeal swab, 95.5% in saliva, and 85.7% in sputum. The nCoV-DK effectively detects SARS-CoV-2 in all types of the samples including saliva, while reducing time required for detection, labor, and risk of human error. The 2019 Novel Coronavirus Detection Kit (nCoV-DK, Shimadzu Corporation, Kyoto, Japan) eliminates the steps of RNA extraction and purification by using the Ampdirect TM technology (Nishimura et al., 2010) , thus significantly reducing the time required for sample preparation and PCR detection from more than 2 hours to about 1 hour. We herein compared efficacy of the nCoV-DK with the direct PCR method requiring RNA extraction and purification. Particularly, it should be noted saliva is a reliable tool to detect the virus by the nCoV-KD even without process of RNA extraction and purification.	./cache/cord-340410-s9haq8y1.txt	./txt/cord-340410-s9haq8y1.txt