id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-339524-r0a6a1jw	Islam, M. T.	A rapid and cost-effective multiplex ARMS-PCR method for the simultaneous genotyping of the circulating SARS-CoV-2 phylogenetic clades	2020-10-13		.txt	text/plain	2518	157	60	title: A rapid and cost-effective multiplex ARMS-PCR method for the simultaneous genotyping of the circulating SARS-CoV-2 phylogenetic clades Here, we propose a rapid, simple and cost-effective amplification-refractory mutation system (ARMS)-based multiplex reverse-transcriptase PCR assay to identify six distinct phylogenetic clades: S, L, V, G, GH, and GR. This approach is applied on 24 COVID-19 positive samples as confirmed by CDC approved real-time PCR assay for SARS-CoV-2. This multiplex ARMS-PCR assay is sample, cost-effective, and convenient that can successfully discriminate the circulating phylogenetic clades of SARS-CoV-2. 137 A set of 15 primers ( Table 1) was designed based on the ARMS for differentiating six major 138 clades of SARS-CoV-2: S, L, V, G, GH, and GR. Hence, the single-variant specific PCRs were able to identify the 228 SARS-CoV-2 positive sample containing GR-clade of the virus. This study proposes a simple and exclusive ARMS-based SNP-discriminating method using 257 conventional PCR to establish multiplex-assays in detecting SARS-CoV-2 mutation clades.	./cache/cord-339524-r0a6a1jw.txt	./txt/cord-339524-r0a6a1jw.txt