id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-338648-5evr2v3r	Shental, Noam	Efficient high-throughput SARS-CoV-2 testing to detect asymptomatic carriers	2020-09-11		.txt	text/plain	5352	274	56	We developed P-BEST, a method for Pooling-Based Efficient SARS-CoV-2 Testing, which identifies all positive subjects within a set of samples using a single round of testing. Here, we developed P-BEST, a method for Pooling-Based Efficient SARS-CoV-2 Testing, using a single-stage nonadaptive group-testing approach, which significantly reduces the number of tests required to identify all positive subjects within a large set of samples. PCR results for each of the pools are provided to the detection algorithm (see Methods), which identifies all positive carriers without the need for an additional testing stage (Fig. 1 , A to C). RNA was extracted from each single sample and pools and was then tested for SARS-CoV-2 using the Seegene COVID-19 diagnostic kit. Using a pooling scheme designed for a carrier rate of ~1%, we showed that our method correctly identified all positive carriers in sets of 384 samples pooled into 48 pools, thereby providing an eightfold reduction in the number of required tests.	./cache/cord-338648-5evr2v3r.txt	./txt/cord-338648-5evr2v3r.txt