id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-335270-edga753o	Lopez-Alvarez, Diana	Genome Sequence of SARS-CoV-2 Isolate Cali-01, from Colombia, Obtained Using Oxford Nanopore MinION Sequencing	2020-06-25		.txt	text/plain	974	70	50	title: Genome Sequence of SARS-CoV-2 Isolate Cali-01, from Colombia, Obtained Using Oxford Nanopore MinION Sequencing We report the genome sequence of a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) isolate obtained from a patient with symptoms of coronavirus disease 2019 (COVID-19) who was infected in Cali, Colombia. We report the coding-complete genome sequence of SARS-CoV-2 isolate Cali-01, obtained from a Colombian patient with no recent record of international travel. First, for quality control and filtering of reads (fragments of 400 to 700 bp), we used the gupplyplex script of the ARTIC Network bioinformatics protocol (https://artic.network/ncov-2019/ncov2019-bioinformatics -sop.html), followed by a reference assembly with minimap2 (6) and Pilon (7), using the sequence of the Wuhan-Hu-1 isolate (GenBank accession number MN908947.3). The genome sequence of SARS-CoV-2 isolate Cali-01 was deposited in GISAID and GenBank under accession numbers EPI_ISL_445219 and MT470219, respectively. The sequencing work was carried out at the Virology Laboratory, Department of Microbiology, of the Universidad del Valle, in Cali.	./cache/cord-335270-edga753o.txt	./txt/cord-335270-edga753o.txt