id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-333763-45dzsn2j	Bestle, Dorothea	TMPRSS2 and furin are both essential for proteolytic activation of SARS-CoV-2 in human airway cells	2020-07-23		.txt	text/plain	8969	441	49	The data demonstrate that efficient inhibition of S cleavage by a combination of TMPRSS2 and furin inhibitors can dramatically block SARS-CoV-2 replication in human airway epithelial cells. In conclusion, our data demonstrate that both TMPRSS2 and furin cleave the SARS-CoV-2 S protein and are essential for efficient virus multicycle replication in Calu-3 human airway cells. Virus titers in supernatants were determined by TCID 50 multicycle replication of SARS-CoV-2 in Calu-3 human airway cells is strongly suppressed by inhibiting TMPRSS2 and furin activity, demonstrating that both proteases are crucial for S activation in these cells. Together, our data indicate that furin and TMPRSS2 cleave S at different sites and that cleavage by both proteases is crucial to render the S protein active for mediating virus entry and membrane fusion (Fig 6) .	./cache/cord-333763-45dzsn2j.txt	./txt/cord-333763-45dzsn2j.txt