id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-333754-copxoyqu	Ma, Hsin-Chieh	Expression and membrane integration of SARS-CoV M protein	2008-04-09		.txt	text/plain	3930	191	60	Full-length SARS-CoV M gene fragment was cloned and expressed as a recombinant protein (221 a.a.) with a C-terminal V5-His tag (29 a.a.) in Vero E6 cells (Fig. 1a) . In addition to the glycosylated and un-glycosylated SARS-CoV M proteins, two smaller protein products (marked by thick line and thin line, respectively) were also detected when M gene was expressed in Vero E6 cells ( Fig. 1a and b) . The protein translated in-frame from the third Met (amino acid 83) could still be detected when the authentic 5 0 -untranslated region of SARS-CoV M gene was included in the expression vector (lane 3 in supplement Fig. 2 ). In this study, SARS-CoV M gene fragment was cloned and expressed as a recombinant protein fused with a C-terminal V5 tag in Vero E6 cells (Fig. 1) . Two other expressed SARS-CoV M protein products with smaller size than the full-length one were also detected in Vero E6 cells (Figs.	./cache/cord-333754-copxoyqu.txt	./txt/cord-333754-copxoyqu.txt