id author title date pages extension mime words sentences flesch summary cache txt cord-332557-qm3qfvry Lau, Susanna K.P. SARS Coronavirus Detection Methods 2005-07-17 .txt text/plain 1388 67 54 Using clinical samples from patients with severe acute respiratory syndrome, we showed that the sensitivities of a quantitative reverse transcription–polymerase chain reaction (80% for fecal samples and 25% for urine samples) were higher than those of the polyclonal (50% and 5%) and monoclonal (35% and 8%) antibody-based nucleocapsid antigen capture enzyme-linked immunosorbent assays. Specimens were tested with polyclonal and monoclonal antibody-based capture ELISAs for SARS-CoV nucleocapsid protein and realtime qRT-PCR. Among the 40 fecal samples from SARS patients, 32 (80%) were positive by qRT-PCR, which was significantly higher than that of the polyclonal (50%) and monoclonal (35%) antibody-based ELISAs (McNemar test, p<0.005 and p<0.001, respectively). Of the 133 urine samples from SARS patients, 33 (25%) were positive by qRT-PCR, which was also significantly higher than that of the polyclonal (5%) and monoclonal (8%) antibody-based ELISAs (McNemar test, p<0.001 for both comparisons). ./cache/cord-332557-qm3qfvry.txt ./txt/cord-332557-qm3qfvry.txt