id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-331878-ww9fu8ey	Gao, Xiaopan	Crystal structure of SARS-CoV-2 papain-like protease	2020-09-02		.txt	text/plain	3510	222	58	The papain-like protease (PLpro) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) plays essential roles in virus replication and immune evasion, presenting a charming drug target. We then determined the structure of SARS-CoV-2 PLpro complex by GRL0617 to 2.6 Å, showing the inhibitor accommodates the S3–S4 pockets of the substrate binding cleft. The initial hydrolysis rate was plotted as the function of inhibitor concentration and the plot was fitted by the equation Y=Bottom+(Top-Bottom)/(1+(X/IC 50 )) using software GraphPad. To gain structural and biochemical insight into SARS-CoV-2 PLpro domain (nsp3 746-1063aa, Supporting Information Fig. S1 ), we expressed two PLpro variants. For example, several crystal structures of SARS-CoV-2 PLpro C111S mutant complexed by various non-covalent inhibitors have been recently deposited in Protein Data Bank (PDB ID: 7JIT, 7JIR and 7JIV), which support our assertion. Structure of papain-like protease from SARS-CoV-2 and its complexes with non-covalent inhibitors	./cache/cord-331878-ww9fu8ey.txt	./txt/cord-331878-ww9fu8ey.txt