id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-330944-54xmnum8	Hsu, You-Ren	Investigation of C-terminal domain of SARS nucleocapsid protein–Duplex DNA interaction using transistors and binding-site models	2014-03-31		.txt	text/plain	3927	248	58	AlGaN/GaN high electron mobility transistors (HEMTs) were used to sense the binding between double stranded DNA (dsDNA) and the severe acute respiratory syndrome coronavirus (SARS-CoV) nucleocapsid protein (N protein). Binding-site models using surface coverage ratios were utilized to analyze the signals from the HEMT-based sensors to extract the dissociation constants and predict the number of binding sites. With a quantitative analysis of the signals from the sensors through binding-site models, we are able to reveal how many binding sites are on the receptor (dsDNA) for certain ligands (SARS-N protein) and what the dissociation constants are for ligand-receptor complexes. The chemical reaction for the surface-immobilized receptor (dsDNA) and the free ligand (SARS-N protein-CTD) in bulk solution can be expressed as the following equations: Therefore, we conclude that for a one-binding-site model, the most significant change of the surface coverage ratio is within the range of the ligand concentration between one order higher and one order lower than the value of the dissociation constant.	./cache/cord-330944-54xmnum8.txt	./txt/cord-330944-54xmnum8.txt