id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-330433-y5dvlcda	Olivieri, Emily R.	Analysis of SARS-CoV Receptor Activity of ACE2 Orthologs	2006		.txt	text/plain	1730	102	60	2 Coronavirus spike-receptor interactions are major determinants of species specificity, and transfection of viral genomic RNA or expression of receptors in nonpermissive cell lines usually results in productive infection. The goals of this study were to determine whether ACE2 transcript is produced by permissive and nonpermissive cells derived from diverse species and to use speciesspecific differences in ACE2 to identify amino acids or other post-translational modifications critical for SARS-CoV binding and/or fusion. Cell lines derived from monkey (VeroE6, pRhMk, and pCMK), human (HRT-18, HEK293T, and Huh-7), mink (Mv1Lu), dog (MDCK), cat (CRFK), hamster (BHK-21), and chicken (CEF) were analyzed for susceptibility to SARS-CoV. This data suggests that dog ACE2 and chicken ACE2 may have amino acid or other differences, which inhibit their function as SARS-CoV receptors. 13 We used multiple sequence alignment of efficient and poor receptors, combined with difference mapping on the hACE2 structure, to identify specific residues or post-translational modifications within ACE2 that may be critical for SARS-CoV entry.	./cache/cord-330433-y5dvlcda.txt	./txt/cord-330433-y5dvlcda.txt