id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-330266-uypjqif7	Firpo, Mason R.	Targeting Polyamines Inhibits Coronavirus Infection by Reducing Cellular Attachment and Entry	2020-09-23		.txt	text/plain	5471	330	51	To determine if coronaviruses are susceptible to polyamine depletion, we treated Vero-E6 cells with doses of DFMO ranging from 750 μM to 5 mM for 4 days prior to infection with SARS-CoV-2 at a multiplicity of infection (MOI) of 0.1 plaque forming units (pfu) per cell. To determine if DFMO-mediated virus restriction was a result of polyamine depletion, we treated BHK-R cells with DFMO and, at the time of infection, supplemented with the individual polyamines putrescine, spermidine, and spermine at 1 μM, a level that did not affect cellular viability ( Figure 2A ). We observed that DFMO reduced viral titers and that supplementation with any of the individual polyamines rescued virus replication to untreated levels ( Figure 2C ). 19 We observed no changes in plaque size ( Figure 3A ) or morphology ( Figure 3B ) with DFMO supplementation to the media, suggesting that DFMO was not sufficiently antiviral when applied to cells after infection and highlighting that DFMO must be applied prophylactically to reduce coronavirus replication.	./cache/cord-330266-uypjqif7.txt	./txt/cord-330266-uypjqif7.txt