id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-328064-7owd28rr	Callahan, Cody J.	Open Development and Clinical Validation of Multiple 3D-Printed Nasopharyngeal Collection Swabs: Rapid Resolution of a Critical COVID-19 Testing Bottleneck	2020-07-23		.txt	text/plain	4879	258	51	We tested PCR compatibility by placing the swab head-downward after breaking it off at the break point, when present (as in a typical NP swab collection), in 3 ml of modified CDC VTM (Hanks' balanced salt solution containing 2% heat-inactivated fetal bovine serum [FBS], 100 g/ml gentamicin, 0.5 g/ml amphotericin B [Fungizone], and 10 mg/liter phenol red [15] ) overnight to allow any PCR-inhibitory material to leach into the medium, spiking 1.5 ml with 200 copies/ml of control SARS-CoV-2 amplicon target (representing 2 times the limit of detection on our system), vortexing, and testing using the Abbott RealTime SARS-CoV-2 assay on an Abbott m2000 RealTime system platform (16) , following the same protocol as for clinical testing (37 cycles, with a cycle threshold [C T ] of Յ31.50 being reported as positive). Control and prototype swabs were placed in separate vials of VTM and transported to the BIDMC Clinical Microbiology Laboratories, where each sample was tested on the Abbott m2000 SARS-CoV-2 RT-PCR platform per the standard clinical protocol.	./cache/cord-328064-7owd28rr.txt	./txt/cord-328064-7owd28rr.txt