id author title date pages extension mime words sentences flesch summary cache txt cord-323389-8vp57c1o Wei, S. Field-deployable, rapid diagnostic testing of saliva samples for SARS-CoV-2. 2020-06-16 .txt text/plain 1563 105 62 We developed an assay that detects single copies of SARS-CoV-2 virus directly from saliva and swab samples in 30 min using a simple, one-step protocol that utilizes only a heat block and microcentrifuge tube prefilled with a mixture containing the necessary reagents and has a sensitivity and specificity of 97% and 100%, respectively. To determine which primer set was most sensitive and specific to SARS-CoV-2, we tested the eight primer sets that we designed, along with previously published primer sets 9, 10 , using serial dilutions of 500 to 0.5 copies of SARS-CoV-2 RNA standard spiked into a 25 μ L RT-LAMP reaction ( Figure 1C ). . https://doi.org/10.1101/2020.06.13.20129841 doi: medRxiv preprint necessary for testing clinical samples ( Figure 1D ). In summary, we developed HP-LAMP, which enables rapid detection of SARS-CoV-2 directly from saliva in 30 min using a simple one-step protocol with a LoD of 2 viral copies per μ L of saliva and a sensitivity and specificity of 97% and 100%, respectively. ./cache/cord-323389-8vp57c1o.txt ./txt/cord-323389-8vp57c1o.txt