id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-322388-c2nymio9	Manopo, Ivanus	Evaluation of a safe and sensitive Spike protein-based immunofluorescence assay for the detection of antibody responses to SARS-CoV	2005-01-31		.txt	text/plain	3546	185	52	To study the diagnostic potential of our Spike protein-based immunoassay based on protein C expressed by recombinant baculovirus in Sf-9 cell, a panel of sera containing 21 SARS-positive samples collected 7-76 days after the onset of SARS symptom, 42 non-SARS serum samples, and 100 normal serum samples were subjected to our Spike protein-based IFA test. The immunoblot showed a 32-kDa band, indicating that protein C expressed in a baculovirus system is antigenic for antibody detection of SARS-CoV infection (represented by Fig. 1a) . Fig. 2 shows representative IFA results in the detection of positive serum samples using our Spike protein-based IFA, compared to the conventional IFA performed by SGH and the commercial SARS IFA kit (EUROIMMUN). In this study, we are the first to show that the immunogenic protein C-based IFA is a sensitive and specific method for detecting SARS-CoV infection.	./cache/cord-322388-c2nymio9.txt	./txt/cord-322388-c2nymio9.txt