id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-322329-zqjiiy4l	Liu, Chunyu	Establishment of a reference panel for the detection of anti-SARS-CoV antibodies	2007-06-30		.txt	text/plain	4227	200	56	In this study, we have expressed and purified severe acute respiratory syndrome (SARS) structural proteins and their fragments and developed indirect enzyme-linked immunosorbent assays (ELISAs) that detect antibodies against the SARS N, N1, N2, S1, SC, S2, and M proteins as well as the human coronavirus OC43 and 229E N proteins. In this study, SARS N and S proteins and their fragments were expressed and purified, and samples from convalescent SARS patients and blood donors were screened with enzyme-linked immunosorbent assays (ELISAs) that were developed with these proteins. IgG and IgM immunoglobulin were detected in the 58 SARS convalescent plasma specimens using an indirect ELISA developed with entire virus antigens. In this study, the indirect ELISAs were developed with different fragments of SARS structure proteins and used to detect antibodies in 58 SARS plasma specimens donated by convalescent patients 3 months after onset of SARS.	./cache/cord-322329-zqjiiy4l.txt	./txt/cord-322329-zqjiiy4l.txt