id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-321854-cy8vyb6j	Ripperger, Tyler J.	Orthogonal SARS-CoV-2 Serological Assays Enable Surveillance of Low Prevalence Communities and Reveal Durable Humoral Immunity.	2020-10-14		.txt	text/plain	3289	193	51	Relative to mild COVID-19 cases, individuals with severe disease exhibited elevated virus-neutralizing titers and antibodies against nucleocapsid (N) and the receptor binding domain (RBD) of spike protein. However, 6.5% of the expanded negative control 149 group displayed RBD reactivity that overlapped with PCR+ individuals (Figure 1B, blue shade) , 150 some of whom may have been early into disease and had not yet generated high levels of 151 antibodies. To quantify the sensitivity of the assay relative to time of diagnosis, we measured 152 antibody levels to RBD and plotted these values against time following SARS-CoV-2 PCR+ 153 confirmation. For 308 both memory and plasma cells, there appears to be a 'sweet spot' of antigen avidity that Taken together, we have reported a highly specific serological assay for SARS-CoV-2 323 exposure that is usable in very low seroprevalence communities, and that returns positive 324 results that are highly co-incident with virus neutralization.	./cache/cord-321854-cy8vyb6j.txt	./txt/cord-321854-cy8vyb6j.txt