id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-320567-7je1i8qd	Muenchhoff, Maximilian	Multicentre comparison of quantitative PCR-based assays to detect SARS-CoV-2, Germany, March 2020	2020-06-18		.txt	text/plain	1759	91	52	The aim of this study was to compare the inter-laboratory and inter-method sensitivity of different RT-PCR assays by providing a blinded, frozen dilution series of a nucleic acid extract of a highly positive biosample to seven different diagnostic laboratories in Germany in March 2020. Digital droplet PCR quantification of the distributed dilution series of nucleic acid eluate of SARS-CoV-2-positive clinical material, Germany, March 2020 The undiluted sample showed between 4,325 and 5,015 SARS-CoV-2 RNA copies per reaction using 5 µL of eluate for the CDC N1, N2, N3 and Charité E protocols, but only 850 and 1,951 RNA copies for the Charité N and P primer/probe combinations ( Figure 1A) , respectively, indicating a lower sensitivity of the latter. Driven by false-negative results for samples with low PCR-positivity using the original Charité RdRp reaction (see below and others [8, 9] ), we compared the primer/ probe sequences with currently available SARS-CoV-2 genomes.	./cache/cord-320567-7je1i8qd.txt	./txt/cord-320567-7je1i8qd.txt