id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-318387-s4d442kx	Wang, Ming	Nanopore target sequencing for accurate and comprehensive detection of SARS-CoV-2 and other respiratory viruses	2020-03-06		.txt	text/plain	4680	304	56	COVID-19 diagnosis relies upon nucleic acid detection; however, current recommended methods exhibit high false-negative rates, low sensitivity, and cannot identify other respiratory virus infections, thereby resulting patient misdiagnosis and impeding epidemic containment. Parallel testing with approved qPCR kits of SARS-CoV-2 and NTS using 61 nucleic acid samples from suspected COVID-19 cases confirmed that NTS identified more infected patients as positive, and could also monitor for mutated nucleic acid sequence or other respiratory virus infection in the test sample. https://doi.org/10.1101/2020.03.04.20029538 doi: medRxiv preprint read numbers of the test sample to those of the negative control (with "0" in the negative control 129 calculated as "1"), we defined that a ratio of ≥10 indicates a positive result for that fragment, 130 scoring 1; ≥3 to 10 fold is inconclusive, scoring 0.4; and <3 is negative, scoring 0. The 4 h sequencing output data (Fig. 4a ) revealed that all 153 19 samples defined as positive by qPCR were recognized SARS-CoV-2-infected by NTS, 154	./cache/cord-318387-s4d442kx.txt	./txt/cord-318387-s4d442kx.txt