id author title date pages extension mime words sentences flesch summary cache txt cord-318239-2sraqm6e Phan, Lan T. Clinical features, isolation, and complete genome sequence of severe acute respiratory syndrome coronavirus 2 from the first two patients in Vietnam 2020-05-28 .txt text/plain 1591 100 54 title: Clinical features, isolation, and complete genome sequence of severe acute respiratory syndrome coronavirus 2 from the first two patients in Vietnam Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the etiologic agent In this report, we describe clinical features, virus isolation, and complete genome sequences from the first two SARS-CoV-2 infections in Vietnam. A suspected case was defined as a person with an acute respiratory tract illness, who reported that he/she had fever and cough (with and without difficulty in breathing) and that within 14 days before the onset of disease, he/she had returned or came from areas where SARS-CoV-2 was spreading or had exposed to a laboratory-confirmed case of Covid-19. NP and OP swab specimens of the patients were collected in a single tube containing 3mL virus transport media and tested for SARS-CoV-2 by real-time RT-PCR assays described previously. Once the CPE was observed under the microscope, cell culture supernatants were harvested, divided into aliquots, tested for the presence of SARS-CoV-2 by real-time RT-PCR assays, and stored at -70°C until virus titration and sequencing were performed. ./cache/cord-318239-2sraqm6e.txt ./txt/cord-318239-2sraqm6e.txt