id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-316859-h8lfmr3e	Mu, Jingfang	SARS-CoV-2-encoded nucleocapsid protein acts as a viral suppressor of RNA interference in cells	2020-04-10		.txt	text/plain	2063	132	59	Previous study has reported that severe acute respiratory syndrome coronavirus (SARS-CoV) nucleocapsid (N) protein displayed a VSR activity in mammalian cells via a cellular reversal-of-silencing assay (Cui et al., 2015) . We first examined whether SARS-CoV-2 N possessed VSR activity via a classic reversal-of-silencing assay, in which enhanced green fluorescent protein (EGFP)-specific shRNA was transfected into EGFP-expressing 293T cells, together with a plasmid encoding SARS-CoV-2 N protein with Flag tag. Our data showed that expression of SARS-CoV-2 N markedly restored the protein level of EGFP ( Figure 1A Because RNAi directly results in the cleavage and degradation of target mRNAs, we further examined the VSR activity of SARS-CoV-2 N using the reversal-of-silencing system via Northern blotting with a digoxin (DIG)-labeled RNA probe targeting EGFP ORF 1-400 nt. Our findings showed that SARS-CoV-2 N can antagonize RNAi in both initiation (i.e., siRNA biogenesis) and effector (i.e., RISC assembly and target RNA cleavage) steps.	./cache/cord-316859-h8lfmr3e.txt	./txt/cord-316859-h8lfmr3e.txt