id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-316788-4x5l2h4d	Ryu, Young Bae	Biflavonoids from Torreya nucifera displaying SARS-CoV 3CL(pro) inhibition	2010-11-15		.txt	text/plain	3222	173	59	Following bioactivity-guided fractionation, eight diterpenoids (1–8) and four biflavonoids (9–12) were isolated and evaluated for SARS-CoV 3CL(pro) inhibition using fluorescence resonance energy transfer analysis. As part of an ongoing investigation of potential SARS-CoV 3CL pro inhibitors from medicinal plants, we performed an initial screen of ethanol extracts of the leaves of Torreya nucifera using a fluorescence resonance energy transfer (FRET) assay. We isolated 12 phytochemicals-eight diterpenoids and four biflavonoids-with SARS-CoV 3CL pro inhibitory activity from the ethanol extracts of the leaves of T. Of the isolated compounds, biflavonoid amentoflavone (9) was identified as a potent inhibitor of SARS-CoV 3CL pro , exhibiting an IC 50 value of 8.3 lM. nucifera, is an effective inhibitor of SARS-CoV 3CL pro and is more effective than the corresponding flavones (apigenin and luteolin) and biflavonoid derivatives containing various numbers of methoxy groups.	./cache/cord-316788-4x5l2h4d.txt	./txt/cord-316788-4x5l2h4d.txt