id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-316623-tv5yyfak	Zhang, Jianmin	Aryl methylene ketones and fluorinated methylene ketones as reversible inhibitors for severe acute respiratory syndrome (SARS) 3C-like proteinase	2008-03-04		.txt	text/plain	4944	258	65	The product was purified by column chromatography (50:50 EtOAc/hexanes) to yield 12a as a solid (16 mg To a solution of 11a (10 mg, 0.045 mmol) in dry THF (5 mL) was added LiHMDS (0.1 mL, 1.0 M solution in THF, 0.1 mmol) over a period of 5 min. The mixture was stirred for 1 h at À78°C, and a solution of NFSi (32 mg, 0.1 mmol) in dry THF (3 mL) was added dropwise over 10 min. After 3 h of stirring at 20°C, the solvent was removed in vacuo and the product was purified by column chromatography on silica gel (25:75 EtOAc/hexanes) to yield 21 as a solid (1.35 g, 68%) Based on our previous modeling studies [10b,23], the three-ringed esters utilize a non-covalent and reversible mechanism of inhibition in a S4-S1 binding mode, by blocking entry of substrates into the active site of SARS-CoV 3CL pro .	./cache/cord-316623-tv5yyfak.txt	./txt/cord-316623-tv5yyfak.txt