id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-316536-jpbfgwhl	Raj, V. Stalin	Dipeptidyl peptidase 4 is a functional receptor for the emerging human coronavirus-EMC	2013-03-13		.txt	text/plain	4501	221	48	Moreover, transient expression of human DPP4 in the non-susceptible COS-7 cells rendered these cells susceptible to binding the hCoV-EMC S1-Fc protein to their surface ( Supplementary Fig. 4) . e, Double-stranded viral RNA (cyan) was detected in hCoV-EMC-infected primary human bronchiolar epithelial cell cultures and appeared to be localized to nonciliated cells that express DPP4 (red). In addition, hCoV-EMC infection of human bronchiolar epithelial cell cultures appeared to be localized to the non-ciliated cells that express DPP4 (Fig. 3e) . COS-7 cells transfected with the human DPP4 expression plasmid, but not with the empty plasmid, were efficiently infected by hCoV-EMC as demonstrated by the presence of viral non-structural proteins in the cells (Fig. 4c) and of viral RNA (Fig. 4d) and infectious virus in the cell supernatants (not shown). S1 receptor-binding domains of hCoV-EMC, SARS-CoV and FIPV fused to the Fc region of human IgG, and soluble versions of DPP4 and ACE2 were expressed and purified as described in the Methods.	./cache/cord-316536-jpbfgwhl.txt	./txt/cord-316536-jpbfgwhl.txt