id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-316179-kmdxltie	Fozouni, P.	Direct detection of SARS-CoV-2 using CRISPR-Cas13a and a mobile phone	2020-09-30		.txt	text/plain	8244	507	58	Here we report the development of an amplification-free CRISPR-Cas13a-based mobile phone assay for direct detection of SARS-CoV-2 from nasal swab RNA extracts. When the SARS-CoV-2 sequence became public in January 2020, we set out to develop a Cas13-based direct-detection assay for viral RNA that would avoid the need for amplification and enable point-of-care testing. We tested the performance of the device for detecting SARS-CoV-2 RNA using the triple-crRNA Cas13a assay and a dilution series with full viral RNA isolated from supernatants of infected Vero CCL-81 cells . (B) RNPs made with crRNA 2 and crRNA 4 individually and in combination (50 nM total RNP concentration for each reaction) were tested against 2.9 x 10 5 copies/µL (480 fM) of SARS-CoV-2 IVT N gene RNA, and compared to fluorescence from no target RNA RNP alone controls ("RNP 2," "RNP 4," and "RNP 2+4").	./cache/cord-316179-kmdxltie.txt	./txt/cord-316179-kmdxltie.txt